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An origami paper-based analytical device for DNA damage analysis.
- Source :
-
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2021 Nov 02; Vol. 57 (87), pp. 11465-11468. Date of Electronic Publication: 2021 Nov 02. - Publication Year :
- 2021
-
Abstract
- Detection and characterization of DNA damage plays a critical role in genotoxicity testing, drug screening, and environmental health. We developed a fully integrated origami paper-based analytical device (oPAD) for measuring DNA damage. This simple device allows on-paper cell lysis, DNA extraction, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) reaction and signal readout with simple operation steps, enabling rapid (within 30 min) and high throughput assessment of multiple DNA damages induced by exogenous chemical agents.
- Subjects :
- Animals
Cell Line
DNA chemistry
DNA Nucleotidylexotransferase chemistry
Fluoresceins chemistry
Fluorescent Dyes chemistry
Immobilized Nucleic Acids analysis
Immobilized Nucleic Acids chemistry
In Situ Nick-End Labeling
Microscopy, Confocal instrumentation
Microscopy, Confocal methods
Microscopy, Fluorescence instrumentation
Microscopy, Fluorescence methods
Zebrafish
DNA analysis
DNA Damage
Paper
Subjects
Details
- Language :
- English
- ISSN :
- 1364-548X
- Volume :
- 57
- Issue :
- 87
- Database :
- MEDLINE
- Journal :
- Chemical communications (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 34651618
- Full Text :
- https://doi.org/10.1039/d1cc05019b