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Primary Isolation of Mycobacterium ulcerans.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2387, pp. 17-28. - Publication Year :
- 2022
-
Abstract
- Primary isolation of Mycobacterium ulcerans is the separation and growth of the bacterium from a mixed population either in clinical specimen or environmental specimen in pure cultures. It is a crucial activity as it can be used to monitor antimicrobial treatment, surveillance for antimicrobial resistance, and molecular epidemiology studies toward understanding pathogen ecology and transmission as well as pathogen biology. The process involves removal of unwanted fast-growing bacteria using 5% oxalic acid, inoculation on Lowenstein-Jensen medium supplemented with glycerol, and incubation at temperatures between 30 °C and 33 °C.<br /> (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Subjects :
- Anti-Infective Agents
Culture Media
Glycerol
Mycobacterium ulcerans
Subjects
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2387
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 34643898
- Full Text :
- https://doi.org/10.1007/978-1-0716-1779-3_3