Back to Search
Start Over
Long Noncoding RNA MIR2187HG Suppresses TBK1-Mediated Antiviral Signaling by Deriving miR-2187-3p in Teleost Fish.
- Source :
-
Journal of virology [J Virol] 2022 Jan 12; Vol. 96 (1), pp. e0148421. Date of Electronic Publication: 2021 Oct 13. - Publication Year :
- 2022
-
Abstract
- Long noncoding RNAs (lncRNAs) function as microregulatory factors that influence gene expression after a variety of pathogenic infections, and they have been extensively studied in the past few years. Although less attention has been paid to lncRNAs in lower vertebrates than in mammals, current studies reveals that lncRNAs play a vital role in fish stimulated by pathogens. Here, we discovered a new lncRNA, termed MIR2187HG, which can function as a precursor of a small RNA, miR-2187-3p, with regulatory functions in the miiuy croaker (Miichthys miiuy). Upon Siniperca chuatsi rhabdovirus (SCRV) virus infection, the expression levels of MIR2187HG were remarkably enhanced. Elevated MIR2187HG expression can act as a pivotally negative regulator that participates in the innate immune response of teleost fish to inhibit the intracellular TANK-binding kinase 1 (TBK1)-mediated antiviral signaling pathways, which can effectively avoid excessive immunity. In addition, we found that SCRV could also utilize MIR2187HG to enhance its own numbers. Our results not only provide evidence regarding the involvement of the lncRNAs in response to viruses in fish but also broaden our understanding of the function of lncRNAs as precursor microRNAs (miRNAs) in teleost fish for the first time. IMPORTANCE SCRV infection upregulates MIR2187HG levels, which in turn suppresses SCRV-triggered type I interferon production, thus promoting viral replication in miiuy croaker. Notably, MIR2187HG regulates the release of miR-2187-3p, and TBK1 is a target of miR-2187-3p. MIR2187HG could acquire from miR-2187-3p the function of inhibiting TBK1 expression and subsequently modulate TBK1-mediated NF-κB and IRF3 signaling. The collective results suggest that the novel regulation mechanism of TBK1-mediated antiviral response during RNA viral infection was regulated by MIR2187HG. Therefore, a new regulation mechanism for lncRNAs to regulate antiviral immune responses in fish is proposed.
- Subjects :
- 3' Untranslated Regions
Adaptor Proteins, Signal Transducing genetics
Animals
Base Sequence
Biomarkers
Disease Resistance genetics
Disease Resistance immunology
Fish Diseases immunology
Gene Expression Profiling
Gene Expression Regulation
Host-Pathogen Interactions immunology
MicroRNAs chemistry
Models, Biological
RNA Interference
RNA, Long Noncoding chemistry
Virus Replication
Adaptor Proteins, Signal Transducing metabolism
Fish Diseases genetics
Fish Diseases virology
Host-Pathogen Interactions genetics
MicroRNAs genetics
RNA, Long Noncoding genetics
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5514
- Volume :
- 96
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 34643431
- Full Text :
- https://doi.org/10.1128/JVI.01484-21