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Microplasma Treatment versus Negative Pressure Therapy for Promoting Wound Healing in Diabetic Mice.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2021 Sep 24; Vol. 22 (19). Date of Electronic Publication: 2021 Sep 24. - Publication Year :
- 2021
-
Abstract
- The delayed healing response of diabetic wounds is a major challenge for treatment. Negative pressure wound therapy (NPWT) has been widely used to treat chronic wounds. However, it usually requires a long treatment time and results in directional growth of wound healing skin tissue. We investigated whether nonthermal microplasma (MP) treatment can promote the healing of skin wounds in diabetic mice. Splint excision wounds were created on diabetic mice, and various wound healing parameters were compared among MP treatment, NPWT, and control groups. Quantitative analysis of the re-epithelialization percentage by detecting Ki67 and DSG1 expression in the extending epidermal tongue (EET) of the wound area and the epidermal proliferation index (EPI) was subsequently performed. Both treatments promoted wound healing by enhancing wound closure kinetics and wound bed blood flow; this was confirmed through histological analysis and optical coherence tomography. Both treatments also increased Ki67 and DSG1 expression in the EET of the wound area and the EPI to enhance re-epithelialization. Increased Smad2/3/4 mRNA expression was observed in the epidermis layer of wounds, particularly after MP treatment. The results suggest that the Smad-dependent transforming growth factor β signaling contributes to the enhancement of re-epithelialization after MP treatment with an appropriate exposure time. Overall, a short-term MP treatment (applied for 30 s twice a day) demonstrated comparable or better efficacy to conventional NPWT (applied for 4 h once a day) in promoting wound healing in diabetic mice. Thus, MP treatment exhibits promise for treating diabetic wounds clinically.
- Subjects :
- Animals
Desmoglein 1 metabolism
In Vitro Techniques
Ki-67 Antigen metabolism
Male
Mice
Mice, Mutant Strains
Nitric Oxide metabolism
Plasma Skin Regeneration methods
RNA, Messenger genetics
RNA, Messenger metabolism
Re-Epithelialization physiology
Regional Blood Flow physiology
Signal Transduction
Skin pathology
Skin physiopathology
Smad Proteins genetics
Smad Proteins metabolism
Transforming Growth Factor beta metabolism
Wound Healing genetics
Diabetes Mellitus, Experimental complications
Diabetes Mellitus, Experimental therapy
Negative-Pressure Wound Therapy methods
Plasma Gases therapeutic use
Skin injuries
Wound Healing physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 22
- Issue :
- 19
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 34638608
- Full Text :
- https://doi.org/10.3390/ijms221910266