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[The Relationship between HIF1α and WTAP Expression Level in t(8;21) Acute Myeloid Leukemia].
- Source :
-
Zhongguo shi yan xue ye xue za zhi [Zhongguo Shi Yan Xue Ye Xue Za Zhi] 2021 Oct; Vol. 29 (5), pp. 1424-1428. - Publication Year :
- 2021
-
Abstract
- Objective: To investigate the relationship between hypoxia inducible factor 1 (HIF1α) and Wilms' tumor 1associating protein (WTAP) expression level in t(8;21) acute myeloid leukemia cells.<br />Methods: The t(8;21) acute myeloid leukemia cell lines, including SKNO-1 and Kasumi-1 were treated by Echinomycin for 24 h, RT-qPCR and Western blot were used to detect the expression levels of WTAP mRNA and the protein. The CoCl <subscript>2</subscript> was used to induce the hypoxia of the cells for 24 h, the expression levels of HIF1α, WTAP protein were detected by Western blot.<br />Results: The expression level of WTAP mRNA and the protein in the echinomycin treated group was significantly lower than those in the control group (P<0.01). The expression level of WTAP protein in the CoCl <subscript>2</subscript> treated group was significantly higher than those in the control group (P<0.05).<br />Conclusion: The inhibition of HIF1-α could down-regulates the expression of WTAP, while the up-regulation of HIF1α could up-regulates the expression of WTAP, which shows that there is a positive correlation of HIF1α and WTAP expression. This result suggesting that HIF1α may be involves in the expression regulation of WTAP gene.
Details
- Language :
- Chinese
- ISSN :
- 1009-2137
- Volume :
- 29
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Zhongguo shi yan xue ye xue za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 34627420
- Full Text :
- https://doi.org/10.19746/j.cnki.issn.1009-2137.2021.05.009