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Generation of Drosophila Heparan Sulfate Mutant Cell Lines from Existing Fly Strains.

Authors :
Nakato E
Bowden N
Nakato H
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2303, pp. 627-636.
Publication Year :
2022

Abstract

Genetic studies using a model organism, Drosophila melanogaster, have been contributing to elucidating the in vivo functions of heparan sulfate proteoglycans (HSPGs). On the other hand, biochemical analysis of Drosophila glycosaminoglycans (GAGs) has been limited, mainly due to the insufficient amount of the material obtained from the animal. Recently, a novel in vitro system has been developed by establishing mutant cell lines for heparan sulfate (HS)-modifying enzyme genes. Metabolic radiolabeling of GAGs allows us to assess uncharacterized features of Drosophila GAGs and the effects of the mutations on HS structures and function. The novel in vitro system will provide us with a direct link between detailed structural information of Drosophila HS and a wealth of knowledge on biological phenotypic data obtained over the last two decades using this animal model.<br /> (© 2022. Springer Science+Business Media, LLC, part of Springer Nature.)

Details

Language :
English
ISSN :
1940-6029
Volume :
2303
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
34626411
Full Text :
https://doi.org/10.1007/978-1-0716-1398-6_47