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Efficient production of isomaltose and isomaltooligosaccharides from starch using 1,4-α-glucan 6-α-glucosyltransferase and isopullulanase.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2021 Nov 24; Vol. 85 (12), pp. 2450-2458. - Publication Year :
- 2021
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Abstract
- We attempted to develop an efficient method for producing isomaltose, a disaccharide consisting of an α-(1→6)-linkage, from starch by combining enzymes of known activity. We found that the combination of 1,4-α-glucan 6-α-glucosyltransferase from Bacillus globisporus N75 and isopullulanase from Aspergillus brasiliensis ATCC 9642 led to the efficient synthesis of isomaltose. Inclusion of isoamylase and cyclomaltodextrin glucanotransferase resulted in increased efficiency, with production yields exceeding 70%. Furthermore, we considered that isomaltooligosaccharides could be synthesized from starch by combining 1,4-α-glucan 6-α-glucosyltransferase from Paenibacillus sp. PP710 and isopullulanase. In reactions that additionally utilized isoamylase and α-amylase, the total concentration of product, which included a series of isomaltooligosaccharides from isomaltose to isomaltodecaose, was 131 m m, and the ratio of 6-linked glucopyranosyl bonds to all bonds was 91.7% at a substrate concentration of 10%. The development of these manufacturing methods will accelerate the industrial production of isomaltose and isomaltooligosaccharides.<br /> (© The Author(s) 2021. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)
- Subjects :
- Oligosaccharides biosynthesis
Oligosaccharides chemistry
Aspergillus enzymology
Aspergillus metabolism
Bacillus enzymology
Isomaltose analogs & derivatives
Isomaltose metabolism
Isomaltose chemistry
Isomaltose biosynthesis
Glucosyltransferases metabolism
Glycoside Hydrolases metabolism
Starch metabolism
Starch chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 85
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 34625793
- Full Text :
- https://doi.org/10.1093/bbb/zbab173