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Endogenous expression and localization of HIS-72::mTurquoise2 in C. elegans .

Authors :
Sloan DE
Bembenek JN
Source :
MicroPublication biology [MicroPubl Biol] 2021 Sep 29; Vol. 2021. Date of Electronic Publication: 2021 Sep 29 (Print Publication: 2021).
Publication Year :
2021

Abstract

To generate a non-red/green fluorescent fusion histone protein in C. elegans , we have generated a C-terminal mTurquoise2-tagged HIS-72 at the endogenous locus using CRISPR. We found that HIS-72::mTurquoise2 localizes in a similar pattern to the previously published HIS-72::GFP strain.<br /> (Copyright: © 2021 by the authors.)

Details

Language :
English
ISSN :
2578-9430
Volume :
2021
Database :
MEDLINE
Journal :
MicroPublication biology
Publication Type :
Academic Journal
Accession number :
34604717
Full Text :
https://doi.org/10.17912/micropub.biology.000471