Back to Search
Start Over
PAK1 Silencing Attenuated Proinflammatory Macrophage Activation and Foam Cell Formation by Increasing PPAR γ Expression.
- Source :
-
Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2021 Sep 23; Vol. 2021, pp. 6957900. Date of Electronic Publication: 2021 Sep 23 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Macrophage polarization in response to environmental cues has emerged as an important event in the development of atherosclerosis. Compelling evidences suggest that P21-activated kinases 1 (PAK1) is involved in a wide variety of diseases. However, the potential role and mechanism of PAK1 in regulation of macrophage polarization remains to be elucidated. Here, we observed that PAK1 showed a dramatically increased expression in M1 macrophages but decreased expression in M2 macrophages by using a well-established in vitro model to study heterogeneity of macrophage polarization. Adenovirus-mediated loss-of-function approach demonstrated that PAK1 silencing induced an M2 macrophage phenotype-associated gene profiles but repressed the phenotypic markers related to M1 macrophage polarization. Additionally, dramatically decreased foam cell formation was found in PAK1 silencing-induced M2 macrophage activation which was accompanied with alternation of marker account for cholesterol efflux or influx from macrophage foam cells. Moderate results in lipid metabolism and foam cell formation were found in M1 macrophage activation mediated by AdshPAK1. Importantly, we presented mechanistic evidence that PAK1 knockdown promoted the expression of PPAR γ , and the effect of macrophage activation regulated by PAK1 silencing was largely reversed when a PPAR γ antagonist was utilized. Collectively, these findings reveal that PAK1 is an independent effector of macrophage polarization at least partially attributed to regulation of PPAR γ expression, which suggested PAK1-PPAR γ axis as a novel therapeutic strategy in atherosclerosis management.<br />Competing Interests: The authors declare that they have no conflicts of interest.<br /> (Copyright © 2021 Wen-Lin Cheng et al.)
- Subjects :
- Adenoviridae genetics
Animals
Foam Cells cytology
Foam Cells metabolism
Genetic Vectors genetics
Genetic Vectors metabolism
Lipopolysaccharides pharmacology
Macrophage Activation
Macrophages cytology
Macrophages drug effects
Macrophages metabolism
Mice
Mice, Knockout
RNA, Small Interfering genetics
RNA, Small Interfering metabolism
p21-Activated Kinases antagonists & inhibitors
p21-Activated Kinases genetics
PPAR gamma metabolism
RNA Interference
p21-Activated Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1942-0994
- Volume :
- 2021
- Database :
- MEDLINE
- Journal :
- Oxidative medicine and cellular longevity
- Publication Type :
- Academic Journal
- Accession number :
- 34603600
- Full Text :
- https://doi.org/10.1155/2021/6957900