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Construction of a urocanic acid-producing strain of Serratia marcescens by transduction.
- Source :
-
Applied and environmental microbiology [Appl Environ Microbiol] 1978 Feb; Vol. 35 (2), pp. 231-6. - Publication Year :
- 1978
-
Abstract
- In Serratia marcescens, the mutation responsible for triazolealanine (TRA) resistance was transferred from a TRA-resistant mutant to a urocanase-less mutant by PS20-mediated transduction. The two crosses were performed using as donors two TRA-resistant mutants, whose phenotypes included increased levels of histidine-biosynthetic enzymes and feedback-insensitive phosphoribosyltransferase. In one cross, TRA-resistant transductants were urocanase-less mutants having only increased levels of the enzymes and barely detectable levels of urocanic acid. In the other cross, the transductants were urocanase-less mutants having both phenotypes of the donor, and most produced high concentrations (10.5 mg/ml) of urocanic acid.
Details
- Language :
- English
- ISSN :
- 0099-2240
- Volume :
- 35
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Applied and environmental microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 345965
- Full Text :
- https://doi.org/10.1128/aem.35.2.231-236.1978