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Transforming growth factor beta gene maps to human chromosome 19 long arm and to mouse chromosome 7.
- Source :
-
Somatic cell and molecular genetics [Somat Cell Mol Genet] 1986 May; Vol. 12 (3), pp. 281-8. - Publication Year :
- 1986
-
Abstract
- Transforming growth factors (TGF) are defined as biologically active polypeptides which reversibly confer the transformed phenotype onto untransformed cultured cells. They have been subdivided into two classes: type alpha and type beta TGFs. TGF-beta acts synergistically with TGF-alpha in inducing phenotypic transformation. TGF-beta can also act as a negative autocrine growth factor. A human 1050-bp EcoRi cDNA fragment was used to map the human locus for TGF-beta by Southern blotting of DNA prepared from 17 human X Chinese hamster somatic cell hybrids. The human-specific restriction fragments segregated with human chromosome 19 in all of 14 informative hybrids. All other human chromosomes were discordant with the TGF-beta bands in at least four hybrids. After in situ hybridization of the tritiated TGF-beta probe to normal human metaphase spreads, 151 silver grains were scored in 54 cells. Of 24 grains over chromosome 19, 16 grains (11%) lay over region 19q13.1----q13.3. Of the 54 cells analyzed, 16 (30%) had label over region 19q13.1----q13.3. Thus, TGFB is assigned to chromosome 19, subbands q13.1----q13.3. The Tgf-beta locus in the mouse was mapped to chromosome 7 by hybridizing a murine cDNA probe to a Chinese hamster X mouse hybrid panel. Human chromosome 19 and proximal mouse chromosome 7 share another four homologous loci.
Details
- Language :
- English
- ISSN :
- 0740-7750
- Volume :
- 12
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Somatic cell and molecular genetics
- Publication Type :
- Academic Journal
- Accession number :
- 3459257
- Full Text :
- https://doi.org/10.1007/BF01570787