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Beta-transforming growth factor is stored in human blood platelets as a latent high molecular weight complex.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1986 Apr 14; Vol. 136 (1), pp. 30-7. - Publication Year :
- 1986
-
Abstract
- Human blood platelets, the richest known source of beta-transforming Growth Factor extractable under acid conditions, release in neutral extracts (pH 7.2) a latent form of this growth factor with an apparent molecular weight of 400 Kd. This latent form, poorly active on rat NRK-49F indicator cells in soft agar assays can be activated by exposure to acid pH or 8 molar urea. The acid activated beta-Transforming Growth Factor from neutral extracts elutes on Biogel P60, in 1 molar acetic acid, as a broad peak of apparent molecular weight 15-30 Kd, like when this factor is extracted from platelets by the usual acid-ethanol procedure. Moreover, beta-Transforming Growth Factor from both acid activated neutral extracts and from acid-ethanol extracts elutes on reverse phase at 30% acetonitrile. We suggest that beta-Transforming Growth Factor is stored in human blood platelets as a poorly active high molecular weight complex which may be dissociated and activated in appropriate in vivo microenvironments.
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 136
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 3458465
- Full Text :
- https://doi.org/10.1016/0006-291x(86)90872-7