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Human milk oligosaccharides alleviate stress-induced visceral hypersensitivity and associated microbiota dysbiosis.
- Source :
-
The Journal of nutritional biochemistry [J Nutr Biochem] 2022 Jan; Vol. 99, pp. 108865. Date of Electronic Publication: 2021 Sep 25. - Publication Year :
- 2022
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Abstract
- Pain-related functional gastrointestinal disorders (FGIDs) are characterized by visceral hypersensitivity (VHS) associated with alterations in the microbiota-gut-brain axis. Since human milk oligosaccharides (HMOs) modulate microbiota, gut and brain, we investigated whether HMOs impact VHS, and explored the role of gut microbiota. To induce VHS, C57BL/6JRj mice received hourly water avoidance stress (WAS) sessions for 10 d, or antibiotics (ATB) for 12 d. Challenged and unchallenged (Sham) animals were fed AIN93M diet (Cont) or AIN93M containing 1% of a 6-HMO mix (HMO6). VHS was assessed by monitoring the visceromotor response to colorectal distension. Fecal microbiome was analyzed by shotgun metagenomics. The effect of HMO6 sub-blends on VHS and nociceptive pathways was further tested using the WAS model. In mice fed Cont, WAS and ATB increased the visceromotor response to distension. HMO6 decreased WAS-mediated electromyographic rise at most distension volumes and overall Area Under Curve (AUC=6.12±0.50 in WAS/HMO6 vs. 9.46±0.50 in WAS/Cont; P<.0001). In contrast, VHS in ATB animals was not improved by HMO6. In WAS, HMO6 promoted most microbiota taxa and several functional pathways associated with low VHS and decreased those associated with high VHS. Among the sub-blends, 2'FL+DFL and LNT+6'SL reduced visceromotor response close to Sham/Cont values and modulated serotoninergic and CGRPα-related pathways. This research further substantiates the capacity of HMOs to modulate the microbiota-gut-brain communication and identifies mitigation of abdominal pain as a new HMO benefit. Ultimately, our findings suggest the value of specific HMO blends to alleviate pain associated FGIDs such as infantile colic or Irritable Bowel Syndrome.<br /> (Copyright © 2021. Published by Elsevier Inc.)
- Subjects :
- Abdominal Pain metabolism
Abdominal Pain microbiology
Abdominal Pain psychology
Animals
Bacteria classification
Bacteria genetics
Bacteria isolation & purification
Bacteria metabolism
Dysbiosis metabolism
Dysbiosis microbiology
Dysbiosis psychology
Feces microbiology
Humans
Male
Mice
Mice, Inbred C57BL
Oligosaccharides analysis
Stress, Psychological
Abdominal Pain diet therapy
Dysbiosis diet therapy
Gastrointestinal Microbiome
Milk, Human metabolism
Oligosaccharides metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-4847
- Volume :
- 99
- Database :
- MEDLINE
- Journal :
- The Journal of nutritional biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 34582967
- Full Text :
- https://doi.org/10.1016/j.jnutbio.2021.108865