Back to Search
Start Over
Distribution and Pathogenicity of Two Cutthroat Trout Virus (CTV) Genotypes in Canada.
- Source :
-
Viruses [Viruses] 2021 Aug 31; Vol. 13 (9). Date of Electronic Publication: 2021 Aug 31. - Publication Year :
- 2021
-
Abstract
- The sole member of the Piscihepevirus genus (family Hepeviridae ) is cutthroat trout virus (CTV) but recent metatranscriptomic studies have identified numerous fish hepevirus sequences including CTV-2. In the current study, viruses with sequences resembling both CTV and CTV-2 were isolated from salmonids in eastern and western Canada. Phylogenetic analysis of eight full genomes delineated the Canadian CTV isolates into two genotypes (CTV-1 and CTV-2) within the Piscihepevirus genus. Hepevirus genomes typically have three open reading frames but an ORF3 counterpart was not predicted in the Canadian CTV isolates. In vitro replication of a CTV-2 isolate produced cytopathic effects in the CHSE-214 cell line with similar amplification efficiency as CTV. Likewise, the morphology of the CTV-2 isolate resembled CTV, yet viral replication caused dilation of the endoplasmic reticulum lumen which was not previously observed. Controlled laboratory studies exposing sockeye ( Oncorhynchus nerka) , pink ( O. gorbuscha ), and chinook salmon ( O.   tshawytscha ) to CTV-2 resulted in persistent infections without disease and mortality. Infected Atlantic salmon ( Salmo salar ) and chinook salmon served as hosts and potential reservoirs of CTV-2. The data presented herein provides the first in vitro and in vivo characterization of CTV-2 and reveals greater diversity of piscihepeviruses extending the known host range and geographic distribution of CTV viruses.
- Subjects :
- Animals
Canada
Genotype
Hepevirus pathogenicity
Persistent Infection virology
Phylogeny
Salmo salar virology
Salmon virology
Trout
Virulence
Viruses, Unclassified classification
Viruses, Unclassified genetics
Viruses, Unclassified isolation & purification
Viruses, Unclassified pathogenicity
Fish Diseases virology
Hepevirus classification
Hepevirus genetics
Hepevirus isolation & purification
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 13
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 34578311
- Full Text :
- https://doi.org/10.3390/v13091730