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Structure of the 4-O-[1-Carboxyethyl]-d-Mannose-Containing O-Specific Polysaccharide of a Halophilic Bacterium Salinivibrio sp. EG9S8QL.

Authors :
Sigida EN
Ibrahim IM
Kokoulin MS
Abulreesh HH
Elbanna K
Konnova SA
Fedonenko YP
Source :
Marine drugs [Mar Drugs] 2021 Sep 07; Vol. 19 (9). Date of Electronic Publication: 2021 Sep 07.
Publication Year :
2021

Abstract

The moderately halophilic strain Salinivibrio sp. EG9S8QL was isolated among 11 halophilic strains from saline mud (Emisal Salt Company, Lake Qarun, Fayoum, Egypt). The lipopolysaccharide was extracted from dried cells of Salinivibrio sp. EG9S8QL by the phenol-water procedure. The OPS was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with <superscript>1</superscript> H and <superscript>13</superscript> C NMR spectroscopy, including <superscript>1</superscript> H, <superscript>1</superscript> H COSY, TOCSY, ROESY, <superscript>1</superscript> H, <superscript>13</superscript> C HSQC, and HMBC experiments. The OPS was found to be composed of linear tetrasaccharide repeating units of the following structure: →2)-β-Man p 4Lac-(1→3)-α-Man p NAc-(1→3)-β-Rha p -(1→4)-α-Glc p NAc-(1→, where Man p 4Lac is 4-O-[1-carboxyethyl]mannose.

Details

Language :
English
ISSN :
1660-3397
Volume :
19
Issue :
9
Database :
MEDLINE
Journal :
Marine drugs
Publication Type :
Academic Journal
Accession number :
34564170
Full Text :
https://doi.org/10.3390/md19090508