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Loop engineering of a thermostable GH10 xylanase to improve low-temperature catalytic performance for better synergistic biomass-degrading abilities.
- Source :
-
Bioresource technology [Bioresour Technol] 2021 Dec; Vol. 342, pp. 125962. Date of Electronic Publication: 2021 Sep 20. - Publication Year :
- 2021
-
Abstract
- Lignocellulosic biorefining for producing biofuels poses technical challenges. It is usually conducted over a long time using heat, making it energy intensive. In this study, we lowered the energy consumption of this process through an optimized enzyme and pretreatment strategy. First, the dominant mutant M137E/N269G of Bispora sp. MEY-1XYL10C&#95;ΔN was obtained by directed evolution with highcatalytic efficiency (970 mL/s∙mg)and specific activity (2090 U/mg)at 37 °C, and thermostability was improved (T <subscript>50</subscript> increased by5 °C). After pretreatment with seawater immersionfollowing steam explosion,bagasse was co-treated with cellulase and M137E/N269G under mild conditions (37 °C), the resulting highest yield of fermentable sugars reached 219 µmol/g of bagasse,46% higher than that of the non-seawater treatment group, with the highest degree of synergy of 2.0. Pretreatment with seawater following steam explosion and synergistic hydrolysis through high activity xylanase and cellulase helped to achieve low energy degradation of lignocellulosic biomass.<br /> (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Subjects :
- Biomass
Hydrolysis
Temperature
Cellulase
Saccharum
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2976
- Volume :
- 342
- Database :
- MEDLINE
- Journal :
- Bioresource technology
- Publication Type :
- Academic Journal
- Accession number :
- 34563821
- Full Text :
- https://doi.org/10.1016/j.biortech.2021.125962