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DEAE-chitosan nanoparticles as a pneumococcus-biomimetic material for the development of antipneumococcal therapeutics.
- Source :
-
Carbohydrate polymers [Carbohydr Polym] 2021 Dec 01; Vol. 273, pp. 118605. Date of Electronic Publication: 2021 Aug 25. - Publication Year :
- 2021
-
Abstract
- Advanced biomaterials provide an interesting and versatile platform to implement new and more effective strategies to fight bacterial infections. Chitosan is one of these biopolymers and possesses relevant features for biomedical applications. Here we synthesized nanoparticles of chitosan derivatized with diethylaminoethyl groups (ChiDENPs) to emulate the choline residues in the pneumococcal cell wall and act as ligands for choline-binding proteins (CBPs). Firstly, we assessed the ability of diethylaminoethyl (DEAE) to sequester the CBPs present in the bacterial surface, thus promoting chain formation. Secondly, the CBP-binding ability of ChiDENPs was purposed to encapsulate a bio-active molecule, the antimicrobial enzyme Cpl-711 (ChiDENPs-711), with improved stability over non-derivatized chitosan. The enzyme-loaded system released more than 90% of the active enzybiotic in ≈ 2 h, above the usual in vivo half-life of this kind of enzymes. Therefore, ChiDENPs provide a promising platform for the controlled release of CBP-enzybiotics in biological contexts.<br /> (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- A549 Cells
Anti-Bacterial Agents chemistry
Bacterial Proteins metabolism
Biomimetic Materials metabolism
Chitosan chemistry
Chitosan metabolism
Drug Carriers metabolism
Drug Liberation
Endopeptidases chemistry
Humans
Nanoparticles metabolism
Streptococcus pneumoniae drug effects
Anti-Bacterial Agents pharmacology
Biomimetic Materials chemistry
Chitosan analogs & derivatives
Drug Carriers chemistry
Endopeptidases pharmacology
Nanoparticles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-1344
- Volume :
- 273
- Database :
- MEDLINE
- Journal :
- Carbohydrate polymers
- Publication Type :
- Academic Journal
- Accession number :
- 34561005
- Full Text :
- https://doi.org/10.1016/j.carbpol.2021.118605