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Influenza Virus Precision Diagnosis and Continuous Purification Enabled by Neuraminidase-Resistant Glycopolymer-Coated Microbeads.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Oct 06; Vol. 13 (39), pp. 46260-46269. Date of Electronic Publication: 2021 Sep 22. - Publication Year :
- 2021
-
Abstract
- Rapid diagnosis and vaccine development are critical to prevent the threat posed by viruses. However, rapid tests, such as colloidal gold assays, yield false-negative results due to the low quantities of viruses; moreover, conventional virus purification, including ultracentrifugation and nanofiltration, is multistep and time-consuming, which limits laboratory research and commercial development of viral vaccines. A rapid virus enrichment and purification technique will improve clinical diagnosis sensitivity and simplify vaccine production. Hence, we developed the surface-glycosylated microbeads (glycobeads) featuring chemically synthetic glycoclusters and reversible linkers to selectively capture the influenza virus. The surface plasmon resonance (SPR) evaluation indicated broad spectrum affinity of S -linked glycosides to various influenza viruses. The magnetic glycobeads were integrated into clinical rapid diagnosis, leading to a 30-fold lower limit of detection. Additionally, the captured viruses can be released under physiological conditions, delivering purified viruses with >50% recovery and without decreasing their native infectivity. Notably, this glycobead platform will facilitate the sensitive detection and continuous one-step purification of the target virus that contributes to future vaccine production.
- Subjects :
- Animals
Carbohydrate Sequence
Chromatography, Affinity
Dogs
HEK293 Cells
Humans
Influenza A Virus, H1N1 Subtype chemistry
Limit of Detection
Madin Darby Canine Kidney Cells
Surface Plasmon Resonance
Influenza A Virus, H1N1 Subtype isolation & purification
Influenza, Human diagnosis
Microspheres
Polysaccharides chemistry
Viral Load methods
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 13
- Issue :
- 39
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 34547894
- Full Text :
- https://doi.org/10.1021/acsami.1c11561