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A reverse vaccinology and immunoinformatics approach for designing a multiepitope vaccine against SARS-CoV-2.
- Source :
-
Immunogenetics [Immunogenetics] 2021 Dec; Vol. 73 (6), pp. 459-477. Date of Electronic Publication: 2021 Sep 20. - Publication Year :
- 2021
-
Abstract
- Since 2019, the world was involved with SARS-CoV-2 and consequently, with the announcement by the World Health Organization that COVID-19 was a pandemic, scientific were an effort to obtain the best approach to combat this global dilemma. The best way to prevent the pandemic from spreading further is to use a vaccine against COVID-19. Here, we report the design of a recombinant multi-epitope vaccine against the four proteins spike or crown (S), membrane (M), nucleocapsid (N), and envelope (E) of SARS-CoV-2 using immunoinformatics tools. We evaluated the most antigenic epitopes that bind to HLA class 1 subtypes, along with HLA class 2, as well as B cell epitopes. Beta-defensin 3 and PADRE sequence were used as adjuvants in the structure of the vaccine. KK, GPGPG, and AAY linkers were used to fuse the selected epitopes. The nucleotide sequence was cloned into pET26b(+) vector using restriction enzymes XhoI and NdeI, and HisTag sequence was considered in the C-terminal of the construct. The results showed that the proposed candidate vaccine is a 70.87 kDa protein with high antigenicity and immunogenicity as well as non-allergenic and non-toxic. A total of 95% of the selected epitopes have conservancy with similar sequences. Molecular docking showed a strong binding between the vaccine structure and tool-like receptor (TLR) 7/8. The docking, molecular dynamics, and MM/PBSA analysis showed that the vaccine established a stable interaction with both structures of TLR7 and TLR8. Simulation of immune stimulation by this vaccine showed that it evokes immune responses related to humoral and cellular immunity.<br /> (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Subjects :
- Amino Acid Sequence
Base Sequence
COVID-19 prevention & control
COVID-19 Vaccines genetics
COVID-19 Vaccines metabolism
Computational Biology
Epitopes, B-Lymphocyte chemistry
Epitopes, B-Lymphocyte genetics
Epitopes, T-Lymphocyte chemistry
Epitopes, T-Lymphocyte genetics
HLA Antigens immunology
Humans
Immunogenicity, Vaccine
Molecular Docking Simulation
Molecular Dynamics Simulation
Molecular Weight
Recombinant Fusion Proteins chemistry
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins immunology
Toll-Like Receptor 7 chemistry
Toll-Like Receptor 8 chemistry
Vaccines, Subunit genetics
Vaccines, Subunit immunology
Vaccines, Subunit metabolism
Vaccinology
Viral Proteins chemistry
Viral Proteins genetics
Viral Proteins immunology
COVID-19 Vaccines immunology
Epitopes, B-Lymphocyte immunology
Epitopes, T-Lymphocyte immunology
SARS-CoV-2 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1211
- Volume :
- 73
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Immunogenetics
- Publication Type :
- Academic Journal
- Accession number :
- 34542663
- Full Text :
- https://doi.org/10.1007/s00251-021-01228-3