Bacterial Survival in Dictyostelium .

Authors :: Rønn R
Hao X
Lüthje F
A German N
Li X
Huang F
Kisaka J
Huffman D
Alwathnani HA
Zhu YG
Rensing C
Source :: Bio-protocol [Bio Protoc] 2017 Jul 05; Vol. 7 (13), pp. e2376. Date of Electronic Publication: 2017 Jul 05 (Print Publication: 2017).
Publication Year :: 2017
Abstract: We performed an assay to test the ability of different E. coli strains to survive inside amoebal cells after ingestion. In the assay we incubated bacteria together with cells of Dictyostelium discoideum for six hours. After co-incubation most of the uningested bacteria were removed by centrifugation and the remaining uningested bacteria were killed by gentamicin. Gentamicin is used because it does not penetrate into eukaryotic cells allowing the ingested bacteria to survive the antibiotic treatment, whereas bacteria outside the amoebal cells are killed.<br /> (Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.)