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Distribution of ciprofloxacin-resistance genes among ST131 and non-ST131 clones of Escherichia coli isolates with ESBL phenotypes isolated from women with urinary tract infection.
- Source :
-
Iranian journal of microbiology [Iran J Microbiol] 2021 Jun; Vol. 13 (3), pp. 294-302. - Publication Year :
- 2021
-
Abstract
- Background and Objectives: Escherichia coli (E. coli) sequence type 131 (ST131) is associated with extended-spectrum beta-lactamase (ESBL) production and fluoroquinolone resistance. This study aimed to investigate the prevalence of ST131, ESBL, and plasmid-mediated quinolone resistance (PMQR) genes in the ciprofloxacin-resistant (CIP <superscript>R</superscript> ) and ESBL producers from women with UTI.<br />Materials and Methods: The CIP-resistant ESBL producing (CIP <superscript>R</superscript> /ESBL <superscript>+</superscript> ) E. coli isolates were screened for ST131-by specific PCR of mdh and gyrB . The ESBL and PMQR genes were screened by single PCR. The ST131 and non-ST131 isolates were selected to determine the mutations of gyrA and parC using PCR and sequencing, and also their genetic background by the Pasteur-MLST scheme.<br />Results: Overall, 55% (33/60) CIP <superscript>R</superscript> /ESBL <superscript>+</superscript> isolates were identified as ST131 (94% O25b-ST131). Resistance rate to ampicillin-sulbactam (70%), aztreonam (97%) and gentamicin (61%), the prevalence of aac(6')-Ib-cr (66%), bla <subscript>CTX-M-15</subscript> (82%), the profile of qnrS+aac(6')-Ib-cr (30%), and the double mutation in the parC was significantly higher in ST131 than non- ST131 isolates. The coexistence of PMQR and ESBL genes was found in more than 50% of ST131 and non-ST131 isolates. ST131 isolates differentiated into PST43 and PST506.<br />Conclusion: Management of women with UTI caused by the CIP <superscript>R</superscript> /ESBL <superscript>+</superscript> isolates (ST131) co-harbored PMQR, ESBL, and chromosomal mutations, is important for their effective therapy.<br /> (Copyright © 2021 The Authors. Published by Tehran University of Medical Sciences.)
Details
- Language :
- English
- ISSN :
- 2008-3289
- Volume :
- 13
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Iranian journal of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 34540167
- Full Text :
- https://doi.org/10.18502/ijm.v13i3.6389