Manipulation of TAD reorganization by chemical-dependent genome linking.

Authors :: Wang J
Ma Q
Fang P
Tian Q
Yu H
Sun J
Ding J
Source :: STAR protocols [STAR Protoc] 2021 Sep 08; Vol. 2 (3), pp. 100799. Date of Electronic Publication: 2021 Sep 08 (Print Publication: 2021).
Publication Year :: 2021
Abstract: Reorganization of topologically associated domain (TAD) is considered to be a novel mechanism for cell fate transitions. Here, we present a protocol to manipulate TAD via abscisic acid (ABA)-dependent genome linking. We use this protocol to merge two adjacent TADs and evaluate the influence on cell fate transitions. The advantages are that the manipulation does not change the genome and is reversible by withdrawing ABA. The major challenge is how to select linking loci for efficient TAD reorganization. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).<br />Competing Interests: The authors declare no competing interests.<br /> (© 2021 The Author(s).)