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Characterization of Integrons and Quinolone Resistance in Clinical Escherichia coli Isolates in Mansoura City, Egypt.
- Source :
-
International journal of microbiology [Int J Microbiol] 2021 Sep 04; Vol. 2021, pp. 6468942. Date of Electronic Publication: 2021 Sep 04 (Print Publication: 2021). - Publication Year :
- 2021
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Abstract
- Escherichia coli is a common pathogen in both humans and animals. Quinolones are used to treat infections caused by Gram-negative bacteria, but resistance genes emerged. Only scarce studies investigated the association between plasmid-mediated quinolone resistance (PMQR) genes and integrons in clinical isolates of E. coli . The current study investigated the prevalence of quinolone resistance and integrons among 134 clinical E. coli isolates. Eighty (59.70%) isolates were quinolone-resistant, and 60/134 (44.77%) isolates were integron positive with the predominance of class I integrons (98.33%). There was a significant association between quinolone resistance and the presence of integrons ( P < 0.0001). Isolates from Urology and Nephrology Center and Gastroenterology Hospital were significantly quinolone-resistant and integron positive ( P ≤ 0.0005). Detection of PMQR genes on plasmids of integron-positive isolates showed that the active efflux pump genes oqxAB and qepA had the highest prevalence (72.22%), followed by the aminoglycoside acetyltransferase gene ( aac(6')-Ib-cr , 66.67%) and the quinolone resistance genes ( qnr , 61.11%). Amplification and sequencing of integrons' variable regions illustrated that no quinolone resistance genes were detected, and the most predominant gene cassettes were for trimethoprim and aminoglycoside resistance including dfrA17, dfrB4 , and dfrA17-aadA5 . In conclusion, this study reported the high prevalence of PMQR genes and integrons among clinical E. coli isolates. Although PMQR genes are not cassette-born, they were associated with integrons' presence, which contributes to the widespread of quinolone resistance in Egypt.<br />Competing Interests: The authors declare that they have no conflict of interest.<br /> (Copyright © 2021 Shaymaa H. Abdel-Rhman et al.)
Details
- Language :
- English
- ISSN :
- 1687-918X
- Volume :
- 2021
- Database :
- MEDLINE
- Journal :
- International journal of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 34527054
- Full Text :
- https://doi.org/10.1155/2021/6468942