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Mechanism of negative membrane curvature generation by I-BAR domains.
- Source :
-
Structure (London, England : 1993) [Structure] 2021 Dec 02; Vol. 29 (12), pp. 1440-1452.e4. Date of Electronic Publication: 2021 Sep 13. - Publication Year :
- 2021
-
Abstract
- The membrane sculpting ability of BAR domains has been attributed to the intrinsic curvature of their banana-shaped dimeric structure. However, there is often a mismatch between this intrinsic curvature and the diameter of the membrane tubules generated. I-BAR domains are especially mysterious since they are almost flat but generate high negative membrane curvature. Here, we use atomistic implicit-solvent computer modeling to show that the membrane bending of the IRSp53 I-BAR domain is dictated by its higher oligomeric structure, whose curvature is completely unrelated to the intrinsic curvature of the dimer. Two other I-BARs give similar results, whereas a flat F-BAR sheet develops a concave membrane-binding interface, consistent with its observed positive membrane curvature generation. Laterally interacting helical spirals of I-BAR dimers on tube interiors are stable and have an enhanced binding energy that is sufficient for membrane bending to experimentally observed tubule diameters at a reasonable surface density.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2021 Elsevier Ltd. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1878-4186
- Volume :
- 29
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Structure (London, England : 1993)
- Publication Type :
- Academic Journal
- Accession number :
- 34520736
- Full Text :
- https://doi.org/10.1016/j.str.2021.07.010