Back to Search Start Over

Fiber-optic label-free biosensor for SARS-CoV-2 spike protein detection using biofunctionalized long-period fiber grating.

Authors :
Lee SL
Kim J
Choi S
Han J
Seo G
Lee YW
Source :
Talanta [Talanta] 2021 Dec 01; Vol. 235, pp. 122801. Date of Electronic Publication: 2021 Aug 13.
Publication Year :
2021

Abstract

With COVID-19 widespread worldwide, people are still struggling to develop faster and more accurate diagnostic methods. Here we demonstrated the label-free detection of SARS-CoV-2 spike protein by employing a SARS-CoV-2 spike antibody-conjugated phase-shifted long-period fiber grating (PS-LPFG) inscribed with a CO <subscript>2</subscript> laser. At a specific cladding mode, the wavelength separation (λ <subscript>D</subscript> ) between the two split dips of a PS-LPFG varies with the external refractive index, although it is virtually insensitive to ambient temperature variations. To detect SARS-CoV-2 spike protein, SARS-CoV-2 spike antibodies were immobilized on the fiber surface of the fabricated PS-LPFG functionalized through chemical modification. When exposed to SARS-CoV-2 spike protein with different concentrations, the antibody-immobilized PS-LPFG exhibited the variation of λ <subscript>D</subscript> according to the protein concentration, which was caused by bioaffinity binding-induced local changes in the refractive index at its surface. In particular, we also confirmed the potential of our sensor for clinical application by detecting SARS-CoV-2 spike protein in virus transport medium. Moreover, our sensor could distinguish SARS-CoV-2 spike protein from those of MERS-CoV and offer efficient properties such as reusability and storage stability. Hence, we have successfully fabricated a promising optical transducer for the detection of SARS-CoV-2 spike protein, which can be unperturbed by external temperature disturbances.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-3573
Volume :
235
Database :
MEDLINE
Journal :
Talanta
Publication Type :
Academic Journal
Accession number :
34517659
Full Text :
https://doi.org/10.1016/j.talanta.2021.122801