Back to Search
Start Over
Cytochrome P450-catalyzed biosynthesis of furanoditerpenoids in the bioenergy crop switchgrass (Panicum virgatum L.).
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 2021 Nov; Vol. 108 (4), pp. 1053-1068. Date of Electronic Publication: 2021 Sep 24. - Publication Year :
- 2021
-
Abstract
- Specialized diterpenoid metabolites are important mediators of plant-environment interactions in monocot crops. To understand metabolite functions in plant environmental adaptation that ultimately can enable crop improvement strategies, a deeper knowledge of the underlying species-specific biosynthetic pathways is required. Here, we report the genomics-enabled discovery of five cytochrome P450 monooxygenases (CYP71Z25-CYP71Z29) that form previously unknown furanoditerpenoids in the monocot bioenergy crop Panicum virgatum (switchgrass). Combinatorial pathway reconstruction showed that CYP71Z25-CYP71Z29 catalyze furan ring addition directly to primary diterpene alcohol intermediates derived from distinct class II diterpene synthase products. Transcriptional co-expression patterns and the presence of select diterpenoids in switchgrass roots support the occurrence of P450-derived furanoditerpenoids in planta. Integrating molecular dynamics, structural analysis and targeted mutagenesis identified active site determinants that contribute to the distinct catalytic specificities underlying the broad substrate promiscuity of CYP71Z25-CYP71Z29 for native and non-native diterpenoids.<br /> (© 2021 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)
- Subjects :
- Biocatalysis
Biological Products chemistry
Biological Products metabolism
Catalytic Domain
Cytochrome P-450 Enzyme System genetics
Diterpenes chemistry
Panicum chemistry
Panicum genetics
Plant Proteins genetics
Plant Proteins metabolism
Plant Roots chemistry
Plant Roots enzymology
Plant Roots genetics
Biosynthetic Pathways
Cytochrome P-450 Enzyme System metabolism
Diterpenes metabolism
Genome, Plant genetics
Panicum enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1365-313X
- Volume :
- 108
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 34514645
- Full Text :
- https://doi.org/10.1111/tpj.15492