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Application of CD54 in diagnosing bone marrow involvement by using flow cytometry in patients with diffuse large B-cell lymphoma.
- Source :
-
BMC cancer [BMC Cancer] 2021 Sep 09; Vol. 21 (1), pp. 1011. Date of Electronic Publication: 2021 Sep 09. - Publication Year :
- 2021
-
Abstract
- Background: Flow cytometry plays a key role in detecting bone marrow (BM) involvement in patients with diffuse large B-cell lymphoma (DLBCL). To improve its detection sensitivity, we need to explore novel markers. In this study, we detected the expression CD54 on lymphoma cells in BM specimens from DLBCL patients and clarified its diagnostic significance in BM involvement by DLBCL.<br />Methods: We collected BM specimens from 76 patients with DLBCL (germinal center B-cell (GCB) = 25, non-GCB = 51) and 10 control patients without lymphoma. We detected and compared the expression of CD54 on lymphoma cells and normal mature B cells by using 10-color panels.<br />Results: Normal plasma cells expressed a higher level of CD54 as compared with hematogones (p < 0.05) and normal mature B cells (p < 0.05). Among 76 patients, 23 of them (GCB = 12, non-GCB = 11) had BM involvement. Lymphoma B cells from 12 cases (GBC = 4, non-GCB = 8) expressed a higher level of CD54 compared to normal mature B cells (p < 0.05). Additionally, lymphoma cells of the non-GCB subtype frequently expressed a higher level of CD54 in comparison to the GCB subtype (p < 0.05). And the high expression of CD54 was not related to plasmacytoid differentiation.<br />Conclusion: Aberrant expression of CD54 on lymphoma cells is frequently seen in patients' BM specimens involved by DLBCL, especially in the non-GCB subtype. CD54 could be used as a new marker to gate on lymphoma cells and improve the detection sensitivity of BM involvement in patients with DLBCL.<br /> (© 2021. The Author(s).)
- Subjects :
- Adult
Aged
Aged, 80 and over
B-Lymphocytes chemistry
B-Lymphocytes metabolism
Biomarkers, Tumor metabolism
Biopsy
Bone Marrow metabolism
Bone Marrow pathology
Cell Adhesion Molecules analysis
Cell Adhesion Molecules metabolism
Cell Differentiation
Female
Germinal Center chemistry
Germinal Center metabolism
Germinal Center pathology
Humans
Immunophenotyping
Intercellular Adhesion Molecule-1 metabolism
Lymphoma, Large B-Cell, Diffuse metabolism
Lymphoma, Large B-Cell, Diffuse pathology
Male
Middle Aged
Plasma Cells cytology
Biomarkers, Tumor analysis
Bone Marrow chemistry
Flow Cytometry
Intercellular Adhesion Molecule-1 analysis
Lymphoma, Large B-Cell, Diffuse chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2407
- Volume :
- 21
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- BMC cancer
- Publication Type :
- Academic Journal
- Accession number :
- 34503477
- Full Text :
- https://doi.org/10.1186/s12885-021-08753-0