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PAF signaling plays a role in obesity-induced adipose tissue remodeling.
- Source :
-
International journal of obesity (2005) [Int J Obes (Lond)] 2022 Jan; Vol. 46 (1), pp. 68-76. Date of Electronic Publication: 2021 Sep 07. - Publication Year :
- 2022
-
Abstract
- Background/objectives: Platelet-activating factor receptor (PAFR) activation controls adipose tissue (AT) expansion in animal models. Our objective was twofold: (i) to check whether PAFR signaling is involved in human obesity and (ii) investigate the PAF pathway role in hematopoietic or non-hematopoietic cells to control adipocyte size.<br />Materials/subjects and Methods: Clinical parameters and adipose tissue gene expression were evaluated in subjects with obesity. Bone marrow (BM) transplantation from wild-type (WT) or PAFR <superscript>-/-</superscript> mice was performed to obtain chimeric PAFR-deficient mice predominantly in hematopoietic or non-hematopoietic-derived cells. A high carbohydrate diet (HC) was used to induce AT remodeling and evaluate in which cell compartment PAFR signaling modulates it. Also, 3T3-L1 cells were treated with PAF to evaluate fat accumulation and the expression of genes related to it.<br />Results: PAFR expression in omental AT from humans with obesity was negatively correlated to different corpulence parameters and more expressed in the stromal vascular fraction than adipocytes. Total PAFR <superscript>-/-</superscript> increased adiposity compared with WT independent of diet-induced obesity. Differently, WT mice receiving PAFR <superscript>-/-</superscript> -BM exhibited similar adiposity gain as WT chimeras. PAFR <superscript>-/-</superscript> mice receiving WT-BM showed comparable augmentation in adiposity as total PAFR <superscript>-/-</superscript> mice, demonstrating that PAFR signaling modulates adipose tissue expansion through non-hematopoietic cells. Indeed, the PAF treatment in 3T3-L1 adipocytes reduced fat accumulation and expression of adipogenic genes.<br />Conclusions: Therefore, decreased PAFR signaling may favor an AT accumulation in humans and animal models. Importantly, PAFR signaling, mainly in non-hematopoietic cells, especially in adipocytes, appears to play a significant role in regulating diet-induced AT expansion.<br /> (© 2021. The Author(s), under exclusive licence to Springer Nature Limited.)
- Subjects :
- Adipose Tissue metabolism
Adult
Animals
Disease Models, Animal
Female
Humans
Male
Mice
Mice, Knockout
Middle Aged
Obesity physiopathology
Paris
Receptors, G-Protein-Coupled
Signal Transduction physiology
Adipose Tissue physiopathology
Obesity complications
Platelet Membrane Glycoproteins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1476-5497
- Volume :
- 46
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of obesity (2005)
- Publication Type :
- Academic Journal
- Accession number :
- 34493775
- Full Text :
- https://doi.org/10.1038/s41366-021-00961-9