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High occurrence of carbapenem-resistant Escherichia coli isolates from healthy rabbits (Oryctolagus cuniculus): first report of bla IMI and bla VIM type genes from livestock in Tunisia.

Authors :
Lengliz S
Benlabidi S
Raddaoui A
Cheriet S
Ben Chehida N
Najar T
Abbassi MS
Source :
Letters in applied microbiology [Lett Appl Microbiol] 2021 Dec; Vol. 73 (6), pp. 708-717. Date of Electronic Publication: 2021 Sep 18.
Publication Year :
2021

Abstract

We aimed to study the antibiotic susceptibility and possible occurrence of extended-spectrum beta-lactamases (ESBL)/carbapenemase-producing Escherichia coli isolates collected from rabbits in Tunisia. In all, 35 faecal samples from healthy rabbits were collected from one farm and E. coli were isolated from three media: antibiotic-free TBX agar, TBX+2 mg l <superscript>-1</superscript> cefotaxime and TBX+1 mg l <superscript>-1</superscript> imipenem. In total, 39 E. coli isolates were recovered; the majority showed resistance to at least one antibiotic and none was ESBL producer. Carbapenem resistance was detected in 16 isolates from either selective or un-selective media. Phenotypic methods used to detect carbapenemase production showed two positive isolates by Modified Hodge Test, six metallo-carbapenemase producers (Imipenem disc+EDTA) and all were temocillin resistant (possible OXA-48 carbapenemase). bla <subscript>VIM</subscript> and bla <subscript>IMP</subscript> type genes were detected in two and one isolates, respectively; one of them harboured both genes. Isolates contained common genes encoding resistance to sulphonamides (sul1, sul2), tetracycline (tetA, tetB, tetC) and fluoroquinolones (qnrS, aac(6')-Ib-cr). Class 1 and 2 integrons were detected in five and four isolates, respectively. These findings highlight the importance of rabbit production as reservoir of carbapenem-resistant E. coli and argument the first report of bla <subscript>VIM</subscript> and bla <subscript>IMP</subscript> genes in livestock in Tunisia.<br /> (© 2021 The Society for Applied Microbiology.)

Details

Language :
English
ISSN :
1472-765X
Volume :
73
Issue :
6
Database :
MEDLINE
Journal :
Letters in applied microbiology
Publication Type :
Academic Journal
Accession number :
34487562
Full Text :
https://doi.org/10.1111/lam.13558