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Robotic sample changers for macromolecular X-ray crystallography and biological small-angle X-ray scattering at the National Synchrotron Light Source II.
- Source :
-
Journal of synchrotron radiation [J Synchrotron Radiat] 2021 Sep 01; Vol. 28 (Pt 5), pp. 1649-1661. Date of Electronic Publication: 2021 Aug 23. - Publication Year :
- 2021
-
Abstract
- Here we present two robotic sample changers integrated into the experimental stations for the macromolecular crystallography (MX) beamlines AMX and FMX, and the biological small-angle scattering (bioSAXS) beamline LiX. They enable fully automated unattended data collection and remote access to the beamlines. The system designs incorporate high-throughput, versatility, high-capacity, resource sharing and robustness. All systems are centered around a six-axis industrial robotic arm coupled with a force torque sensor and in-house end effectors (grippers). They have the same software architecture and the facility standard EPICS-based BEAST alarm system. The MX system is compatible with SPINE bases and Unipucks. It comprises a liquid nitrogen dewar holding 384 samples (24 Unipucks) and a stay-cold gripper, and utilizes machine vision software to track the sample during operations and to calculate the final mount position on the goniometer. The bioSAXS system has an in-house engineered sample storage unit that can hold up to 360 samples (20 sample holders) which keeps samples at a user-set temperature (277 K to 300 K). The MX systems were deployed in early 2017 and the bioSAXS system in early 2019.
Details
- Language :
- English
- ISSN :
- 1600-5775
- Volume :
- 28
- Issue :
- Pt 5
- Database :
- MEDLINE
- Journal :
- Journal of synchrotron radiation
- Publication Type :
- Academic Journal
- Accession number :
- 34475312
- Full Text :
- https://doi.org/10.1107/S1600577521007578