Back to Search
Start Over
Sorting for secreted molecule production using a biosensor-in-microdroplet approach.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Sep 07; Vol. 118 (36). - Publication Year :
- 2021
-
Abstract
- Sorting large libraries of cells for improved small molecule secretion is throughput limited. Here, we combine producer/secretor cell libraries with whole-cell biosensors using a microfluidic-based screening workflow. This approach enables a mix-and-match capability using off-the-shelf biosensors through either coencapsulation or pico-injection. We demonstrate the cell type and library agnostic nature of this workflow by utilizing single-guide RNA, transposon, and ethyl-methyl sulfonate mutagenesis libraries across three distinct microbes ( Escherichia coli , Saccharomyces cerevisiae , and Yarrowia lipolytica ), biosensors from two organisms ( E. coli and S. cerevisiae ), and three products (triacetic acid lactone, naringenin, and L-DOPA) to identify targets improving production/secretion.<br />Competing Interests: Competing interest statement: X.L. and F.F.C. are employees of Sphere Fluidics Limited.
- Subjects :
- Biosensing Techniques
Escherichia coli genetics
Escherichia coli metabolism
Fluorescence
Levodopa biosynthesis
Mutagenesis
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae metabolism
Yarrowia genetics
Yarrowia metabolism
High-Throughput Screening Assays methods
Microfluidics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1091-6490
- Volume :
- 118
- Issue :
- 36
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 34475218
- Full Text :
- https://doi.org/10.1073/pnas.2106818118