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Arachnoid membrane as a source of sphingosine-1-phosphate that regulates mouse middle cerebral artery tone.
- Source :
-
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism [J Cereb Blood Flow Metab] 2022 Jan; Vol. 42 (1), pp. 162-174. Date of Electronic Publication: 2021 Sep 02. - Publication Year :
- 2022
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Abstract
- Growing evidence indicates that perivascular tissue is critical to modulate vessel function. We hypothesized that the arachnoid membrane surrounding middle cerebral artery (MCA) regulates its function via sphingosine-1-phosphate (S1P)-induced vasoconstriction. The MCA from 3- to 9-month-old male and female wild-type (Oncine France 1 and C57BL/6) mice and sphingosine kinase 2 knockout (SphK2-/-) mice in the C57BL/6 background was mounted in pressure myographs with and without arachnoid membrane. Raman microspectroscopy and imaging were used for in situ detection of S1P. The presence of arachnoid tissue was associated with reduced external and lumen MCA diameters, and with an increase in basal tone regardless of sex and strain background. Strong S1P-positive signals were detected in the arachnoid surrounding the MCA wall in both mice models, as well as in a human post-mortem specimen. Selective S1P receptor 3 antagonist TY 52156 markedly reduced both MCA vasoconstriction induced by exogenous S1P and arachnoid-dependent basal tone increase. Compared to 3-month-old mice, the arachnoid-mediated contractile influence persisted in 9-month-old mice despite a decline in arachnoid S1P deposits. Genetic deletion of SphK2 decreased arachnoid S1P content and vasoconstriction. This is the first experimental evidence that arachnoid membrane regulates the MCA tone mediated by S1P.
- Subjects :
- Animals
Female
Hydrazones pharmacology
Lysophospholipids genetics
Male
Mice
Mice, Knockout
Phosphotransferases (Alcohol Group Acceptor) deficiency
Phosphotransferases (Alcohol Group Acceptor) metabolism
Sphingosine genetics
Sphingosine metabolism
Sphingosine-1-Phosphate Receptors antagonists & inhibitors
Sphingosine-1-Phosphate Receptors genetics
Arachnoid metabolism
Lysophospholipids metabolism
Middle Cerebral Artery metabolism
Signal Transduction
Sphingosine analogs & derivatives
Sphingosine-1-Phosphate Receptors metabolism
Vasoconstriction
Subjects
Details
- Language :
- English
- ISSN :
- 1559-7016
- Volume :
- 42
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
- Publication Type :
- Academic Journal
- Accession number :
- 34474613
- Full Text :
- https://doi.org/10.1177/0271678X211033362