Triplex Direct Quantitative Polymerase Chain Reaction for the Identification of Streptococcus pneumoniae Serotypes.

Authors :: Ouattara M
Tamboura M
Kambiré D
Lê KA
Van Phan T
Velusamy S
Nguyen HA
Trang DVT
Lessa FC
Iijima M
Nguyen DT
Schwartz SB
McGee L
Traoré RO
Beall B
Source :: The Journal of infectious diseases [J Infect Dis] 2021 Sep 01; Vol. 224 (12 Suppl 2), pp. S204-S208.
Publication Year :: 2021
Abstract: The quantitative polymerase chain reaction (qPCR) method presented in this study allows the identification of pneumococcal capsular serotypes in cerebrospinal fluid without first performing DNA extraction. This testing approach, which saves time and resources, demonstrated similar sensitivity and a high level of agreement between cycle threshold values when it was compared side-by-side with the standard qPCR method with extracted DNA.<br /> (Published by Oxford University Press for the Infectious Diseases Society of America 2021.)

Subjects :: Humans
Serogroup
Serotyping
Streptococcus pneumoniae isolation & purification
Multiplex Polymerase Chain Reaction methods
Pneumococcal Infections diagnosis
Streptococcus pneumoniae genetics

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