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Interleukin-1 blockade attenuates white matter inflammation and oligodendrocyte loss after progressive systemic lipopolysaccharide exposure in near-term fetal sheep.
- Source :
-
Journal of neuroinflammation [J Neuroinflammation] 2021 Aug 31; Vol. 18 (1), pp. 189. Date of Electronic Publication: 2021 Aug 31. - Publication Year :
- 2021
-
Abstract
- Background: Increased systemic and tissue levels of interleukin (IL)-1β are associated with greater risk of impaired neurodevelopment after birth. In this study, we tested the hypothesis that systemic IL-1 receptor antagonist (Ra) administration would attenuate brain inflammation and injury in near-term fetal sheep exposed to lipopolysaccharide (LPS).<br />Methods: Chronically instrumented near-term fetal sheep at 0.85 of gestation were randomly assigned to saline infusion (control, n = 9), repeated LPS infusions (0 h = 300 ng, 24 h = 600 ng, 48 h = 1200 ng, n = 8) or repeated LPS plus IL-1Ra infusions (13 mg/kg infused over 4 h) started 1 h after each LPS infusion (n = 9). Sheep were euthanized 4 days after starting infusions for histology.<br />Results: LPS infusions increased circulating cytokines and were associated with electroencephalogram (EEG) suppression with transiently reduced mean arterial blood pressure, and increased carotid artery perfusion and fetal heart rate (P < 0.05 vs. control for all). In the periventricular and intragyral white matter, LPS-exposure increased IL-1β immunoreactivity, numbers of caspase 3+ cells and microglia, reduced astrocyte and olig-2+ oligodendrocyte survival but did not change numbers of mature CC1+ oligodendrocytes, myelin expression or numbers of neurons in the cortex and subcortical regions. IL-1Ra infusions reduced circulating cytokines and improved recovery of EEG activity and carotid artery perfusion. Histologically, IL-1Ra reduced microgliosis, IL-1β expression and caspase-3+ cells, and improved olig-2+ oligodendrocyte survival.<br />Conclusion: IL-1Ra improved EEG activity and markedly attenuated systemic inflammation, microgliosis and oligodendrocyte loss following LPS exposure in near-term fetal sheep. Further studies examining the long-term effects on brain maturation are now needed.<br /> (© 2021. The Author(s).)
- Subjects :
- Animals
Brain metabolism
Brain pathology
Encephalitis metabolism
Encephalitis pathology
Female
Interleukin 1 Receptor Antagonist Protein therapeutic use
Oligodendroglia metabolism
Oligodendroglia pathology
Pregnancy
Sheep
White Matter metabolism
White Matter pathology
Brain drug effects
Encephalitis drug therapy
Interleukin 1 Receptor Antagonist Protein pharmacology
Lipopolysaccharides pharmacology
Oligodendroglia drug effects
White Matter drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1742-2094
- Volume :
- 18
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of neuroinflammation
- Publication Type :
- Academic Journal
- Accession number :
- 34465372
- Full Text :
- https://doi.org/10.1186/s12974-021-02238-4