In vitro Histone H3 Cleavage Assay for Yeast and Chicken Liver H3 Protease.

Authors :: Chauhan S
Azad GK
Tomar RS
Source :: Bio-protocol [Bio Protoc] 2017 Jan 05; Vol. 7 (1), pp. e2085. Date of Electronic Publication: 2017 Jan 05 (Print Publication: 2017).
Publication Year :: 2017
Abstract: Histone proteins are subjected to a wide array of reversible and irreversible post-translational modifications (PTMs) (Bannister and Kouzarides, 2011; Azad and Tomar, 2014). The PTMs on histones are known to regulate chromatin structure and function. Histones are irreversibly modified by proteolytic clipping of their tail domains. The proteolytic clipping of histone tails is continuously attracting interest of researchers in the field of chromatin biology. We can recapitulate H3-clipping by performing in vitro H3 cleavage assay. Here, we are presenting the detailed protocol to perform in vitro H3 cleavage assay.<br /> (Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.)