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Melatonin Inhibits Annulus Fibrosus Cell Senescence through Regulating the ROS/NF- κ B Pathway in an Inflammatory Environment.
- Source :
-
BioMed research international [Biomed Res Int] 2021 Aug 18; Vol. 2021, pp. 3456321. Date of Electronic Publication: 2021 Aug 18 (Print Publication: 2021). - Publication Year :
- 2021
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Abstract
- Inflammation response is an important reason for disc cell senescence during disc degeneration. Recently, melatonin is suggested to protect against disc degeneration. However, the effects of melatonin on annulus fibrosus (AF) cell senescence are not fully studied. The main purpose of this study was to investigate the effects of melatonin on AF cell senescence in an inflammatory environment and the underlying mechanism. Rat disc AF cells were cultured in a medium with tumor necrosis factor- α (TNF- α ). Melatonin was added along with the medium to observe its protective effects. Compared with the control AF cells, TNF- α significantly declined cell proliferation potency and telomerase activity, elevated senescence-associated β -galactosidase (SA- β -Gal) activity, upregulated protein expression of senescence markers (p16 and p53), and increased reactive oxygen species (ROS) content and activity of the NF- κ B pathway. However, when the TNF- α -treated AF cells were incubated with melatonin, ROS content and activity of the NF- κ B pathway were decreased, and those parameters reflecting cell senescence indicated that AF cell senescence was also partly alleviated. Together, melatonin suppresses AF cell senescence through regulating the ROS/NF- κ B pathway in an inflammatory environment. This study sheds a new light that melatonin may be promising to retard inflammation-caused disc degeneration.<br />Competing Interests: There are no conflicts of interest in this work.<br /> (Copyright © 2021 Jing Li et al.)
- Subjects :
- Animals
Annulus Fibrosus metabolism
Annulus Fibrosus pathology
Antioxidants pharmacology
Cells, Cultured
Cellular Senescence drug effects
Inflammation metabolism
Inflammation pathology
Intervertebral Disc Degeneration metabolism
Intervertebral Disc Degeneration pathology
Male
Models, Animal
NF-kappa B genetics
Rats
Rats, Sprague-Dawley
Telomerase metabolism
Tumor Necrosis Factor-alpha metabolism
Annulus Fibrosus drug effects
Inflammation drug therapy
Intervertebral Disc Degeneration drug therapy
Melatonin pharmacology
NF-kappa B metabolism
Reactive Oxygen Species metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2314-6141
- Volume :
- 2021
- Database :
- MEDLINE
- Journal :
- BioMed research international
- Publication Type :
- Academic Journal
- Accession number :
- 34458366
- Full Text :
- https://doi.org/10.1155/2021/3456321