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miR-634 inhibits human vascular smooth muscle cell proliferation and migration in hypertension through Wnt4/β-catenin pathway.
- Source :
-
Frontiers in bioscience (Landmark edition) [Front Biosci (Landmark Ed)] 2021 Aug 30; Vol. 26 (8), pp. 395-404. - Publication Year :
- 2021
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Abstract
- MicroRNAs (miRNAs) have been regarded as modulators in vascular pathologies, including hypertension. Dysregulated proliferation and migration of VSMCs (vascular smooth muscle cells) contributes to vascular remodeling during hypertension. miR-634 was reported to be dysregulated in hypertensive patients. The involvement of miR-634 in hypertension and the role of miR-634 on VSMCs proliferation and migration were then evaluated. Firstly, HASMCs (human aortic smooth muscle cells) were incubated with 2 μM angiotensin (Ang) II for 12 hours to establish the cell model of Ang II-induced hypertension. Results showed that Ang II treatment promoted proliferation and migration of HASMCs. Secondly, miR-634 was down-regulated in the hypertensive patients, and reduced in Ang II-induced HASMCs in a time dependent manner. Functional assays revealed that Ang II promoted proliferation and migration of HASMCs were suppressed by miR-634 mimic. Lastly, miR-634 targeted 3' untranslated region (UTR) of Wnt4, and reduced Wnt4 expression in HASMCs. miR-634 inhibited β-catenin nuclear translocation. Over-expression of Wnt4 counteracted the suppressive effects of miR-634 on Ang II-induced proliferation and migration of HASMCs. In conclusion, miR-634 inhibited HASMCs proliferation and migration through inactivation of Wnt4/β-catenin pathway.<br /> (© 2021 The Author(s). Published by BRI.)
Details
- Language :
- English
- ISSN :
- 2768-6698
- Volume :
- 26
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Frontiers in bioscience (Landmark edition)
- Publication Type :
- Academic Journal
- Accession number :
- 34455769
- Full Text :
- https://doi.org/10.52586/4953