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Tracheal aspirate RNA sequencing identifies distinct immunological features of COVID-19 ARDS.
- Source :
-
Nature communications [Nat Commun] 2021 Aug 26; Vol. 12 (1), pp. 5152. Date of Electronic Publication: 2021 Aug 26. - Publication Year :
- 2021
-
Abstract
- The immunological features that distinguish COVID-19-associated acute respiratory distress syndrome (ARDS) from other causes of ARDS are incompletely understood. Here, we report the results of comparative lower respiratory tract transcriptional profiling of tracheal aspirate from 52 critically ill patients with ARDS from COVID-19 or from other etiologies, as well as controls without ARDS. In contrast to a "cytokine storm," we observe reduced proinflammatory gene expression in COVID-19 ARDS when compared to ARDS due to other causes. COVID-19 ARDS is characterized by a dysregulated host response with increased PTEN signaling and elevated expression of genes with non-canonical roles in inflammation and immunity. In silico analysis of gene expression identifies several candidate drugs that may modulate gene expression in COVID-19 ARDS, including dexamethasone and granulocyte colony stimulating factor. Compared to ARDS due to other types of viral pneumonia, COVID-19 is characterized by impaired interferon-stimulated gene (ISG) expression. The relationship between SARS-CoV-2 viral load and expression of ISGs is decoupled in patients with COVID-19 ARDS when compared to patients with mild COVID-19. In summary, assessment of host gene expression in the lower airways of patients reveals distinct immunological features of COVID-19 ARDS.<br /> (© 2021. The Author(s).)
- Subjects :
- Adult
Aged
Aged, 80 and over
COVID-19 immunology
COVID-19 virology
Case-Control Studies
Cohort Studies
Critical Illness
Cytokines genetics
Cytokines immunology
Female
Gene Expression Profiling
Humans
Male
Middle Aged
RNA metabolism
Respiratory Distress Syndrome immunology
Respiratory Distress Syndrome virology
SARS-CoV-2 physiology
Sequence Analysis, RNA
COVID-19 genetics
RNA genetics
Respiratory Distress Syndrome genetics
Trachea immunology
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 34446707
- Full Text :
- https://doi.org/10.1038/s41467-021-25040-5