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Cytotoxic Minor Piericidin Derivatives from the Actinomycete Strain Streptomyces psammoticus SCSIO NS126.
- Source :
-
Marine drugs [Mar Drugs] 2021 Jul 28; Vol. 19 (8). Date of Electronic Publication: 2021 Jul 28. - Publication Year :
- 2021
-
Abstract
- The mangrove-sediment-derived actinomycete strain Streptomyces &nbsp; psammoticus SCSIO NS126 was found to have productive piericidin metabolites featuring anti-renal cell carcinoma activities. In this study, in order to explore more diverse piericidin derivatives, and therefore to discover superior anti-tumor lead compounds, the NS126 strain was further fermented at a 300-L scale under optimized fermentation conditions. As a result, eight new minor piericidin derivatives (piericidins L-R ( 1 - 7 ) and 11-demethyl-glucopiericidin A ( 8 )) were obtained, along with glucopiericidin B ( 9 ). The new structures including absolute configurations were determined by spectroscopic methods coupled with experimental and calculated electronic circular dichroism. We also proposed plausible biosynthetic pathways for these unusual post-modified piericidins. Compounds 1 and 6 showed selective cytotoxic activities against OS-RC-2 cells, and 2 - 5 exhibited potent cytotoxicity against HL-60 cells, with IC <subscript>50</subscript> values lower than 0.1 μM. The new piericidin glycoside 8 was cytotoxic against ACHN, HL-60 and K562, with IC <subscript>50</subscript> values of 2.3, 1.3 and 5.5 μM, respectively. The ability to arrest the cell cycle and cell apoptosis effects induced by 1 and 6 in OS-RC-2 cells, 2 in HL-60 cells, and 8 in ACHN cells were then further investigated. This study enriched the structural diversity of piericidin derivatives and confirmed that piericidins deserve further investigations as promising anti-tumor agents.
Details
- Language :
- English
- ISSN :
- 1660-3397
- Volume :
- 19
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Marine drugs
- Publication Type :
- Academic Journal
- Accession number :
- 34436267
- Full Text :
- https://doi.org/10.3390/md19080428