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Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration.

Authors :
Arts JJ
Mahlandt EK
Grönloh ML
Schimmel L
Noordstra I
Gordon E
van Steen AC
Tol S
Walzog B
van Rijssel J
Nolte MA
Postma M
Khuon S
Heddleston JM
Wait E
Chew TL
Winter M
Montanez E
Goedhart J
van Buul JD
Source :
ELife [Elife] 2021 Aug 25; Vol. 10. Date of Electronic Publication: 2021 Aug 25.
Publication Year :
2021

Abstract

Upon inflammation, leukocytes rapidly transmigrate across the endothelium to enter the inflamed tissue. Evidence accumulates that leukocytes use preferred exit sites, alhough it is not yet clear how these hotspots in the endothelium are defined and how they are recognized by the leukocyte. Using lattice light sheet microscopy, we discovered that leukocytes prefer endothelial membrane protrusions at cell junctions for transmigration. Phenotypically, these junctional membrane protrusions are present in an asymmetric manner, meaning that one endothelial cell shows the protrusion and the adjacent one does not. Consequently, leukocytes cross the junction by migrating underneath the protruding endothelial cell. These protrusions depend on Rac1 activity and by using a photo-activatable Rac1 probe, we could artificially generate local exit-sites for leukocytes. Overall, we have discovered a new mechanism that uses local induced junctional membrane protrusions to facilitate/steer the leukocyte escape/exit from inflamed vessel walls.<br />Competing Interests: JA, EM, MG, LS, IN, EG, Av, ST, BW, Jv, MN, MP, SK, JH, EW, TC, MW, EM, JG, Jv No competing interests declared<br /> (© 2021, Arts et al.)

Details

Language :
English
ISSN :
2050-084X
Volume :
10
Database :
MEDLINE
Journal :
ELife
Publication Type :
Academic Journal
Accession number :
34431475
Full Text :
https://doi.org/10.7554/eLife.66074