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Endothelial junctional membrane protrusions serve as hotspots for neutrophil transmigration.
- Source :
-
ELife [Elife] 2021 Aug 25; Vol. 10. Date of Electronic Publication: 2021 Aug 25. - Publication Year :
- 2021
-
Abstract
- Upon inflammation, leukocytes rapidly transmigrate across the endothelium to enter the inflamed tissue. Evidence accumulates that leukocytes use preferred exit sites, alhough it is not yet clear how these hotspots in the endothelium are defined and how they are recognized by the leukocyte. Using lattice light sheet microscopy, we discovered that leukocytes prefer endothelial membrane protrusions at cell junctions for transmigration. Phenotypically, these junctional membrane protrusions are present in an asymmetric manner, meaning that one endothelial cell shows the protrusion and the adjacent one does not. Consequently, leukocytes cross the junction by migrating underneath the protruding endothelial cell. These protrusions depend on Rac1 activity and by using a photo-activatable Rac1 probe, we could artificially generate local exit-sites for leukocytes. Overall, we have discovered a new mechanism that uses local induced junctional membrane protrusions to facilitate/steer the leukocyte escape/exit from inflamed vessel walls.<br />Competing Interests: JA, EM, MG, LS, IN, EG, Av, ST, BW, Jv, MN, MP, SK, JH, EW, TC, MW, EM, JG, Jv No competing interests declared<br /> (© 2021, Arts et al.)
- Subjects :
- Animals
Cell Line
Green Fluorescent Proteins
Human Umbilical Vein Endothelial Cells
Humans
Male
Mice
Mice, Transgenic
Microscopy, Electron, Transmission
Muscle, Skeletal physiology
Muscle, Skeletal ultrastructure
Gene Expression Regulation physiology
Intercellular Junctions physiology
Neutrophils physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2050-084X
- Volume :
- 10
- Database :
- MEDLINE
- Journal :
- ELife
- Publication Type :
- Academic Journal
- Accession number :
- 34431475
- Full Text :
- https://doi.org/10.7554/eLife.66074