Enzymatic determination of d-alanine with l-alanine dehydrogenase and alanine racemase.

Authors :: Ashida H
Sawa Y
Yoshimura T
Source :: Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2021 Oct 21; Vol. 85 (11), pp. 2221-2223.
Publication Year :: 2021
Abstract: An enzymatic assay system of d-Ala, which is reported to affect the taste, was constructed using alanine racemase and l-alanine dehydrogenase. d-Ala is converted to l-Ala by alanine racemase and then deaminated by l-alanine dehydrogenase with the reduction of NAD+ to NADH, which is determined with water-soluble tetrazolium. Using the assay system, the d-Ala contents of 7 crustaceans were determined.<br /> (© The Author(s) 2021. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)

Subjects :: Animals
NAD metabolism
Enzyme Assays methods
Alanine Racemase metabolism
Alanine Racemase genetics
Alanine Dehydrogenase metabolism
Alanine Dehydrogenase genetics
Alanine Dehydrogenase chemistry
Alanine metabolism
Alanine analysis

Full Text Access

Tools