Description of Lujinxingia vulgaris sp. nov., isolated from coastal sediment via prey-traps.

Two Gram-stain negative, facultative anaerobic, oxidase-negative, catalase-positive bacilli, designated as strains TMQ4 ^T and TMQ2, were isolated from Xiaoshi Island, China, using prey-traps. Growth was observed within the ranges 25-45 °C (optimally at 37 °C), pH 6.5-9.0 (optimally at pH 7.5-8.0) and 1-8% NaCl (optimally at 3-4%, w/v). The draft genome sequences of strains TMQ4 ^T and TMQ2 contained 184 contigs of 5,609,735 bp with a G+C content of 64.4% and 148 contigs of 5,589,985 bp with a G+C content of 65.0%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that both strains belonged to the genus Lujinxingia with the similarity of 98.9%. The phylogenetic and phylogenomic topologies and analyses demonstrated that both strains clustered together and differentiated from the closest neighbour, Lujinxingia sediminis SEH01 ^T . Genomic analyses showed that two strains lost the biosynthesis pathway of several chemical compounds. Iso-C _15:0 was contained in the predominant cellular fatty acids in both strains. The major polar lipids of both strains consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and unidentified lipids; and the respiratory quinone was menaquinone MK-7 for both strains. Both strains predated other bacteria, including Owenweeksia hongkongensis JCM 12287 ^T and Paraliobacillus ryukyuensis DSM 15140 ^T , and were lured with one prey Acinetobacter baumannii ATCC 19606 ^T in prey-trap. Combining genomic analyses, two strains had the predatory indices of 2, similar to representative typical bacterial predators. The physiological, biochemical, and phylogenetic properties suggest that the two strains represent a novel species within the genus Lujinxingia. The name Lujinxingia vulgaris sp. nov. is proposed, with strain TMQ4 ^T (= KCTC 62851 ^T  = MCCC 1H00392 ^T ) as type strain and strain TMQ2 (= KCTC 72,079 = MCCC 1H00381) as reference strain.
(© 2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)