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Clog-free high-throughput microfluidic cell isolation with multifunctional microposts.
- Source :
-
Scientific reports [Sci Rep] 2021 Aug 17; Vol. 11 (1), pp. 16685. Date of Electronic Publication: 2021 Aug 17. - Publication Year :
- 2021
-
Abstract
- Microfluidics have been applied to filtration of rare tumor cells from the blood as liquid biopsies. Processing is highly limited by low flow rates and device clogging due to a single function of fluidic paths. A novel method using multifunctional hybrid functional microposts was developed. A swift by-passing route for non-tumor cells was integrated to prevent very common clogging problems. Performance was characterized using microbeads (10 µm) and human cancer cells that were spiked in human blood. Design-I showed a capture efficiency of 96% for microbeads and 87% for cancer cells at 1 ml/min flow rate. An improved Design-II presented a higher capture efficiency of 100% for microbeads and 96% for cancer cells. Our method of utilizing various microfluidic functions of separation, bypass and capture has successfully guaranteed highly efficient separation of rare cells from biological fluids.<br /> (© 2021. The Author(s).)
Details
- Language :
- English
- ISSN :
- 2045-2322
- Volume :
- 11
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Scientific reports
- Publication Type :
- Academic Journal
- Accession number :
- 34404819
- Full Text :
- https://doi.org/10.1038/s41598-021-94123-6