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The Performance of Immunoassays to Measure Antibodies to the Chlamydia trachomatis Antigen Pgp3 in Different Epidemiological Settings for Trachoma.
- Source :
-
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2021 Aug 16; Vol. 105 (5), pp. 1362-1367. Date of Electronic Publication: 2021 Aug 16. - Publication Year :
- 2021
-
Abstract
- Programs to eliminate trachoma as a public health problem use prevalence of the clinical sign trachomatous inflammation-follicular (TF) in 1- to 9-year-olds in endemic districts to make decisions to begin or end mass drug administration with azithromycin. Trachomatous inflammation-follicular is used as a proxy for transmission of ocular Chlamydia trachomatis infection. Long-term monitoring of previously endemic districts for recrudescence of ocular C. trachomatis infection would benefit from a simple blood test that could be integrated with other public health programs. In this study, we evaluated multiple tests to measure antibodies against the C. trachomatis antigen Pgp3-a multiplex bead assay (MBA), an ELISA, and two versions of a lateral flow assay (LFA)-in four districts of the Amhara region of Ethiopia with varying levels of TF. Seroprevalence and seroconversion rate (SCR) results were proportional to TF prevalence by district for most tests, with the notable exception of the LFA using colloidal gold as the developing reagent. Changing the test developing reagent to black latex improved agreement between serological measures and TF prevalence and in inter-rater agreement. Seroconversion rate estimates using data derived from the LFA-gold assay were inconsistent with the shape of the age-seroprevalence curve, which did not increase in older ages. These data revealed potential complications with using SCR that will need further evaluation. Data from MBA, ELISA, and LFA with the black test line showed good agreement with each other and proportionality to TF estimates, providing further data that serology has potential utility for trachoma surveillance.
- Subjects :
- Anti-Bacterial Agents therapeutic use
Child
Child, Preschool
Ethiopia epidemiology
Female
Humans
Immunologic Tests methods
Infant
Male
Prevalence
Seroepidemiologic Studies
Trachoma epidemiology
Antibodies, Bacterial blood
Antigens, Bacterial blood
Azithromycin therapeutic use
Chlamydia trachomatis immunology
Chlamydia trachomatis isolation & purification
Trachoma diagnosis
Trachoma immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1476-1645
- Volume :
- 105
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- The American journal of tropical medicine and hygiene
- Publication Type :
- Academic Journal
- Accession number :
- 34398819
- Full Text :
- https://doi.org/10.4269/ajtmh.21-0541