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2003-2019: explosive spread of enterobacteria producing extended-spectrum beta-lactamases in Bangui Central African Republic.
- Source :
-
The Pan African medical journal [Pan Afr Med J] 2021 May 07; Vol. 39, pp. 22. Date of Electronic Publication: 2021 May 07 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Introduction: the spread of enterobacteria producing extended-broad-spectrum beta-lactamases (ESBL) is a global public health-problem. In a study carried in 2003-2005 at the Pasteur Institute in Bangui, 450 enterobacteria were identified in clinical isolates, of which 17 were ESBL (prevalence: 3.78%). The aim of this study was to update this data.<br />Methods: from May 2018 to April 2019, a total of 941 enterobacteria were isolated and identified under identical conditions of recruitment and with the same techniques used in the previous study: phenotypic identification using Api 20E strips (bioMérieux SA, Marcy-l'Etoile, France) and antimicrobial drug susceptibility using the disk diffusion method (Bio-Rad antibiotic discs, Marnes la Coquette, France). Resistance genes were identified by polymerase chain reaction (PCR) and sequencing.<br />Results: from May 2018 to April 2019, a total of 941 enterobacteria were isolated of which 478 were ESBL, thus amounting to a prevalence of 50.80%. The genetic profiles of the bla CTX-M resistance genes exhibited the emergence of the CTX-M28 variant (CTX-M1 group) and variants of the M2 and M9 groups. There was also a notable increase, from 35 to 64%, in the ESBL with a bla SHV gene.<br />Conclusion: this study documents a 13 fold increase in the prevalence of ESBL derived from clinical isolates of the bacteriology laboratory of the Institute Pasteur in Bangui, by comparing its data with that of the publication by Frank et al. 2006. Together with this increase a significant diversification of the circulating CTX-M resistance genes was noticed.<br />Competing Interests: The authors declare no competing interests.<br /> (Copyright: Hugues Sanke-Waïgana et al.)
- Subjects :
- Bacterial Proteins genetics
Central African Republic epidemiology
Disk Diffusion Antimicrobial Tests
Drug Resistance, Bacterial genetics
Enterobacteriaceae drug effects
Enterobacteriaceae enzymology
Enterobacteriaceae Infections microbiology
Humans
Polymerase Chain Reaction
Retrospective Studies
Anti-Bacterial Agents pharmacology
Enterobacteriaceae isolation & purification
Enterobacteriaceae Infections epidemiology
beta-Lactamases genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1937-8688
- Volume :
- 39
- Database :
- MEDLINE
- Journal :
- The Pan African medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 34394813
- Full Text :
- https://doi.org/10.11604/pamj.2021.39.22.28812