PPR-DYW Protein EMP17 Is Required for Mitochondrial RNA Editing, Complex III Biogenesis, and Seed Development in Maize.

The conversion of cytidines to uridines (C-to-U) at specific sites in mitochondrial and plastid transcripts is a post-transcriptional processing event that is important to the expression of organellar genes. Pentatricopeptide repeat (PPR) proteins are involved in this process. In this study, we report the function of a previously uncharacterized PPR-DYW protein, Empty pericarp17 (EMP17), in the C-to-U editing and kernel development in maize. EMP17 is targeted to mitochondria. The loss-function of EMP17 arrests maize kernel development, abolishes the editing at ccmF _C -799 and nad2 -677 sites, and reduces the editing at ccmF _C -906 and -966 sites. The absence of editing causes amino acid residue changes in CcmF _C -267 (Ser to Pro) and Nad2-226 (Phe to Ser), respectively. As CcmF _C functions in cytochrome c (Cyt c ) maturation, the amount of Cyt c and Cyt c ₁ protein is drastically reduced in emp17 , suggesting that the CcmF _C -267 (Ser to Pro) change impairs the CcmF _C function. As a result, the assembly of complex III is strikingly decreased in emp17 . In contrast, the assembly of complex I appears less affected, suggesting that the Nad2-226 (Phe to Ser) change may have less impact on Nad2 function. Together, these results indicate that EMP17 is required for the C-to-U editing at several sites in mitochondrial transcripts, complex III biogenesis, and seed development in maize.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Wang, Liu, Huang, Li, Yang, Sayyed, Sun, Gu, Wang and Tan.)