Back to Search
Start Over
First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region.
First evidence of enterovirus A71 and echovirus 30 in Uruguay and genetic relationship with strains circulating in the South American region.
- Source :
-
PloS one [PLoS One] 2021 Aug 12; Vol. 16 (8), pp. e0255846. Date of Electronic Publication: 2021 Aug 12 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Human enteroviruses (EVs) comprise more than 100 types of coxsackievirus, echovirus, poliovirus and numbered enteroviruses, which are mainly transmitted by the faecal-oral route leading to diverse diseases such as aseptic meningitis, encephalitis, and acute flaccid paralysis, among others. Since enteroviruses are excreted in faeces, wastewater-based epidemiology approaches are useful to describe EV diversity in a community. In Uruguay, knowledge about enteroviruses is extremely limited. This study assessed the diversity of enteroviruses through Illumina next-generation sequencing of VP1-amplicons obtained by RT-PCR directly applied to viral concentrates of 84 wastewater samples collected in Uruguay during 2011-2012 and 2017-2018. Fifty out of the 84 samples were positive for enteroviruses. There were detected 27 different types belonging to Enterovirus A species (CVA2-A6, A10, A16, EV-A71, A90), Enterovirus B species (CVA9, B1-B5, E1, E6, E11, E14, E21, E30) and Enterovirus C species (CVA1, A13, A19, A22, A24, EV-C99). Enterovirus A71 (EV-A71) and echovirus 30 (E30) strains were studied more in depth through phylogenetic analysis, together with some strains previously detected by us in Argentina. Results unveiled that EV-A71 sub-genogroup C2 circulates in both countries at least since 2011-2012, and that the C1-like emerging variant recently entered in Argentina. We also confirmed the circulation of echovirus 30 genotypes E and F in Argentina, and reported the detection of genotype E in Uruguay. To the best of our knowledge this is the first report of the EV-A71 C1-like emerging variant in South-America, and the first report of EV-A71 and E30 in Uruguay.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Capsid Proteins genetics
Capsid Proteins metabolism
Enterovirus A, Human classification
Enterovirus A, Human isolation & purification
Enterovirus B, Human classification
Enterovirus B, Human isolation & purification
Enterovirus C, Human classification
Enterovirus C, Human genetics
Enterovirus C, Human isolation & purification
Genotype
Humans
Phylogeny
RNA, Viral chemistry
RNA, Viral genetics
RNA, Viral metabolism
Seasons
South America
Uruguay
Wastewater virology
Enterovirus A, Human genetics
Enterovirus B, Human genetics
Genetic Linkage genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 16
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 34383835
- Full Text :
- https://doi.org/10.1371/journal.pone.0255846