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Identification of differentially expressed microRNAs in primary esophageal achalasia by next-generation sequencing.
- Source :
-
Turkish journal of biology = Turk biyoloji dergisi [Turk J Biol] 2021 Jun 23; Vol. 45 (3), pp. 262-274. Date of Electronic Publication: 2021 Jun 23 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Molecular knowledge regarding the primary esophageal achalasia is essential for the early diagnosis and treatment of this neurodegenerative motility disorder. Therefore, there is a need to find the main microRNAs (miRNAs) contributing to the mechanisms of achalasia. This study was conducted to determine some patterns of deregulated miRNAs in achalasia. This case-control study was performed on 52 patients with achalasia and 50 nonachalasia controls. The miRNA expression profiling was conducted on the esophageal tissue samples using the next-generation sequencing (NGS). Differential expression of miRNAs was analyzed by the edgeR software. The selected dysregulated miRNAs were additionally confirmed using the quantitative reverse transcription polymerase chain reaction (qRT-PCR). Fifteen miRNAs were identified that were significantly altered in the tissues of the patients with achalasia. Among them, three miRNAs including miR-133a-5p, miR-143-3p, and miR-6507-5p were upregulated. Also, six miRNAs including miR-215-5p, miR-216a-5p, miR-216b-5p, miR-217, miR-7641 and miR-194-5p were downregulated significantly. The predicted targets for the dysregulated miRNAs showed significant disease-associated pathways like neuronal cell apoptosis, neuromuscular balance, nerve growth factor signaling, and immune response regulation. Further analysis using qRT-PCR showed significant down-regulation of hsa-miR-217 (p-value = 0.004) in achalasia tissue. Our results may serve as a basis for more future functional studies to investigate the role of candidate miRNAs in the etiology of achalasia and their application in the diagnosis and probably treatment of the disease.<br />Competing Interests: CONFLICT OF INTEREST: The authors declare that they have no competing interests.<br /> (Copyright © 2021 The Author(s).)
Details
- Language :
- English
- ISSN :
- 1303-6092
- Volume :
- 45
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Turkish journal of biology = Turk biyoloji dergisi
- Publication Type :
- Academic Journal
- Accession number :
- 34377051
- Full Text :
- https://doi.org/10.3906/biy-2101-61