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Elicitation of Neutralizing Antibody Responses to HIV-1 Immunization with Nanoparticle Vaccine Platforms.
- Source :
-
Viruses [Viruses] 2021 Jul 02; Vol. 13 (7). Date of Electronic Publication: 2021 Jul 02. - Publication Year :
- 2021
-
Abstract
- A leading strategy for developing a prophylactic HIV-1 vaccine is the elicitation of antibodies that can neutralize a large fraction of circulating HIV-1 variants. However, a major challenge that has limited the effectiveness of current vaccine candidates is the extensive global diversity of the HIV-1 envelope protein (Env), the sole target for HIV-neutralizing antibodies. To address this challenge, various strategies incorporating Env diversity into the vaccine formulation have been proposed. Here, we assessed the potential of two such strategies that utilize a nanoparticle-based vaccine platform to elicit broadly neutralizing antibody responses. The nanoparticle immunogens developed here consisted of different formulations of Envs from strains BG505 (clade A) and CZA97 (clade C), attached to the N-termini of bacterial ferritin. Single-antigen nanoparticle cocktails, as well as mosaic nanoparticles bearing both Env trimers, elicited high antibody titers in mice and guinea pigs. Furthermore, serum from guinea pigs immunized with nanoparticle immunogens achieved autologous, and in some cases heterologous, tier 2 neutralization, although significant differences between mosaic and single-antigen nanoparticles were not observed. These results provide insights into the ability of different vaccine strategies for incorporating Env sequence diversity to elicit neutralizing antibodies, with implications for the development of broadly protective HIV-1 vaccines.
- Subjects :
- AIDS Vaccines administration & dosage
Animals
Antibodies, Neutralizing biosynthesis
Female
Guinea Pigs
HIV Infections immunology
HIV-1 classification
HIV-1 genetics
Immunization
Mice
Mice, Inbred BALB C
Nanoparticles chemistry
env Gene Products, Human Immunodeficiency Virus classification
env Gene Products, Human Immunodeficiency Virus genetics
AIDS Vaccines immunology
Antibodies, Neutralizing immunology
HIV Antibodies blood
HIV Infections prevention & control
HIV-1 immunology
Nanoparticles administration & dosage
env Gene Products, Human Immunodeficiency Virus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 13
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 34372503
- Full Text :
- https://doi.org/10.3390/v13071296