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GP.MOT: A novel glycophorin variant identified in a Japanese blood donor.

Authors :
Oda A
Suzuki Y
Isa K
Ogasawara K
Yabe R
Kimura T
Uchikawa M
Tsuno NH
Source :
Transfusion [Transfusion] 2021 Oct; Vol. 61 (10), pp. 2825-2829. Date of Electronic Publication: 2021 Aug 09.
Publication Year :
2021

Abstract

Background: In this study, we identified a novel glycophorin variant (GP.MOT) in a Mi <superscript>a</superscript> -positive Japanese blood donor. The proband with this glycophorin variant was discovered by antigen screening of samples from 475,493 Japanese blood donors using monoclonal anti-Mi <superscript>a</superscript> .<br />Study Design and Methods: Standard serological techniques and flow cytometry were performed. GP.MOT RBCs were examined by immunoblotting using anti-GPA, anti-MUT or anti-Mur. Genome DNA was extracted from whole blood, and the GYPA/GYPB was analyzed by polymerase chain reactions and Sanger sequencing.<br />Results: The MNS blood group of the proband was M + N +  <superscript>w</superscript> S-s + with the presence of other low-frequency antigens including Mi <superscript>a</superscript> , Mur, MUT, and KIPP. A 43-kDa molecule, which is almost equivalent in size to glycophorin A (GPA), was identified by immunoblotting using monoclonal anti-MUT and anti-Mur. Sanger sequencing clearly indicated that the proband had two different GYPA*M alleles at SNP rs62334651 (GYPA*M232 + 55A and GYPA*M232 + 55G), as well as a GYP(B-A) hybrid allele (GYP*MOT) with breakpoints located on pseudoexon 3 of GYPB from c.210 to c.219.<br />Discussion: We identified a hybrid glycophorin GP.MOT with the deduced unique amino acid sequence GPB (20-45)-GPΨB (46-70)-GPA (71-149), which has not been previously reported.<br /> (© 2021 AABB.)

Details

Language :
English
ISSN :
1537-2995
Volume :
61
Issue :
10
Database :
MEDLINE
Journal :
Transfusion
Publication Type :
Academic Journal
Accession number :
34369596
Full Text :
https://doi.org/10.1111/trf.16535